Coding

Part:BBa_K2027055:Design

Designed by: Gordon Sun   Group: iGEM16_Stanford-Brown   (2016-10-24)


H. Brasiliensis HRT2 cis-prenyltransferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence has been acquired from H. brasiliensis and codon optimized for transfection into E. coli.

The stop codon has been removed from this sequence to allow for C terminus concatenation with tags.

No other modifications have been made to the sequence.

Source

Sequence was sourced from Asawatreratanakul K, Zhang YW, Wititsuwannakul D, et al. Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis. A key factor participating in natural rubber biosynthesis. Eur J Biochem. 2003;270(23):4671-80. (http://www.ncbi.nlm.nih.gov/nuccore/AB064661?report=GenBank)

References